编号 zgly0000340129

文献类型 期刊论文

文献题名 马尾松表达序列标签多态性初步分析

学科分类 220.99;林学其他学科

作者 尹佟明  李东  陈颖  黄敏仁  王明庥 

作者单位 南京林业大学林木遗传与基因工程开放研究实验室南京 

母体文献 林业科学 

年卷期 2004,40(6)

页码 176-180

年份 2004 

分类号 S791.248  Q943 

关键词 马尾松  表达序列标签  单链构象多态性  遗传图谱 

文摘内容 We reported our preliminary study on mapping of the expressed sequence tag (EST) using single-stranded DNA conformation polymorphism (SSCP) on polymerase chain reaction (PCR) product. Five ESTs primer pairs, developed from nuclear genes including CAD ( cinnamyl-alcohol dehydrgenase), CHS ( chalcone synthase), NIR ( nitrite reductase), ARA554(cDNA expressed in differentiating xylem in Pinus taedae ) and GS (glutamine synthetase), were selected to optimize the experimental protocol and generate mapping markers in the megagemtophytes from a single tree of Pinus massoniana. Our ultimate goal was to use SSCP approach to construct a transcriptional map for comparative mapping studies in P. nasoniana.The efficiency to construct a transcriptional map in P. massoniana based on the published EST primer pairs derived from other pine species critically depended on the successful amplification of EST fragments and the ratio of the heterozygous loci revealed in P.massoniana. In this study, 3 (60%) out of 5 tested EST primer pairs were succeeded in amplification, however, only 1 (20%) gene was heterozygous in the tested tree. The ratio of heterozygous loci detected in this study was similar to that revealed by anonymous marker in P. taeda. Therefore, a low efficiency would be expected if the map would be constructed using single pedigree. The segregation ratio of loci revealed by primer pairs would be higher if multiple pedigrees would be used. We proposed that consensus mapping approach based on multiple-pedigree should be used for EST mapping and therefore to increase the efficiency of EST mapping。